Description
Description: This assay employs a two-site sandwich ELISA to quantitate HTR1A in samples. An antibody specific for HTR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The receptor is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin (5-hydroxtryptamine, 5-HT). It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor. The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the central nervous system, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus in high densities, while low amounts also exist in the basal ganglia and thalamus. The 5-HT1A receptors in the raphe nucleus are largely somatodendritic autoreceptors. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan